VINScreen COT Rapid Test Cassette
VINScreen COT Rapid Test Cassette
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VINScreen COT – Cotinine (Nicotine Metabolite) Rapid Urine Test Cassette
Single-panel urine cassette for rapid qualitative detection of Cotinine — the primary metabolite of nicotine — at a cut-off of 500 ng/mL. Ideal for determining smoking status in workplace wellness, insurance, and pre-employment programs.
Panel & Cut-Off
COT (Cotinine) — 500 ng/mL
Detects the primary metabolite of nicotine with a detection window of up to 2–3 days after nicotine use.
What It Detects
Cotinine from all nicotine sources — cigarettes, e-cigarettes/vapes, nicotine patches, gum, nasal sprays and secondhand smoke exposure above the cut-off threshold.
Best For
Insurance health assessments, smoke-free workplace programs, pre-employment screening, smoking cessation monitoring, and healthcare or wellness programs.
Performance & Accuracy
| Metric | Result |
|---|---|
| Overall accuracy vs GC/MS | 98.8% |
| Positive agreement | 97.1% |
| Negative agreement | >99.9% |
| Read time | 5 minutes |
| Sample type | Urine |
| Sensitivity (cut-off) | 500 ng/mL |
Note: Screening results are preliminary. Any positive result should be confirmed by a secondary analytical method such as GC/MS as required by your program policy.
How to Use
- Allow the test cassette and urine specimen to reach room temperature (15–30°C).
- Remove the cassette from the sealed pouch and place on a clean, level surface.
- Hold the dropper vertically and transfer 3 full drops of urine (approx. 120 µL) to the specimen well (S).
- Start the timer. Avoid trapping air bubbles in the specimen well.
- Read results at 5 minutes. Do not interpret results after 10 minutes.
How to Interpret Results
- Negative: Two lines appear — one in the Control region (C) and one in the Test region (T). Even a faint test line is a negative result. Cotinine is below 500 ng/mL.
- Positive: One line appears in the Control region (C) only. No line in the Test region (T). Cotinine exceeds 500 ng/mL.
- Invalid: No Control line appears. Retest with a new cassette.
What's Included
- VINScreen COT Rapid Test Cassettes
- Droppers
- Package Insert / Instructions for Use
Specimen collection container and timer are required but not included.
Storage & Shelf Life
- Store in sealed pouch at 2–30°C (room temperature or refrigerated).
- Stable through the expiration date printed on the sealed pouch.
- Do not freeze. Do not use after the expiration date.
Product Specifications
- Brand: VINScreen
- Test: COT (Cotinine / Nicotine Metabolite)
- Format: Single-panel urine test cassette
- Cut-Off: 500 ng/mL
- Read Time: 5 minutes
- Sample: Urine
- Detection Window: Up to 2–3 days after nicotine use
- Catalogue #: RP5802340
- Use: For forensic use only — determination of smoking status
FAQ
What is Cotinine and why test for it?
Cotinine is the primary metabolite of nicotine and is considered the most reliable biological marker for determining tobacco/nicotine use. Unlike nicotine itself, cotinine has a stable elimination profile unaffected by urine pH, making it ideal for screening.
Will nicotine replacement therapy (NRT) cause a positive result?
Yes. Nicotine patches, gum, lozenges and nasal sprays all produce cotinine. This test detects cotinine from any nicotine source above the 500 ng/mL cut-off.
Can secondhand smoke cause a positive result?
Casual secondhand exposure is unlikely to reach the 500 ng/mL cut-off. However, prolonged or heavy secondhand exposure may produce detectable levels in some cases.
How long is cotinine detectable in urine?
At the 500 ng/mL cut-off, cotinine is typically detectable for up to 2–3 days after the last nicotine use, depending on individual metabolism, frequency of use and hydration.
Full Description
Full Description
Specification
Specification
